Prevalence of Porphyromonas gingivalis fimA II genotype in generalized aggressive periodontitis patients

Purpose: The objective of this study was to evaluate the prevalence of Porphyromonas gingivalis (Pg) and its filmA II genotype in a sample of Brazilian patients with generalized aggressive periodontitis (GAgP) and to correlate the presence of each pathogen/genotype eith clinical parameters. Methods: We used polymerase chain reaction (PCR) to evaluate the presence of Pg and filmA II genotype in subgingival plaque samples collected from the deepest site of 45 Brazilian patients aged 15-40 years with GAgP and correlated findings with age and clinical parameters (plaque index, gingival bleeding index, probing depth and clinical attachment loss). Results: Pg was identified in 64.4% patients. FilmA II genotype was present in 82.6% of Pg-positive patients. The presence of Pg and filmA II genotype was significantly associated with greater clinical attachment loss at the sampled periodontal site. Pg-positive patients were slightly older than Pg-negative patients. Conclusions: Pg and filmA II genotype were highly prevalente in Brazilian patients with GAgP. Pg was more commonly observed in slightly older individuals and in sites with more clinical attachment loss. (AU)

Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis

Abstract The objective of this study was to investigate the association between single nucleotide polymorphisms (SNPs) in the IL10, NOS2A, and ESR2 genes and chronic periodontitis (CP) and aggressive periodontitis (AgP). Three groups of patients underwent periodontal and radiographic evaluations: CP (n = 61), AgP (n = 50), and periodontally healthy (control group=61). Genomic DNA was extracted from oral epithelial cells and used for genotyping by real-time polymerase chain reaction using TaqMan® probes. The investigated SNPs were: -1087G > A, -819C > T and -592C > A in the IL10; +2087G > A in the NOS2A, and +1730G > A in the ESR2 gene. Differences in genotype and allele frequencies of each polymorphism and some individual characteristics were analyzed using the chi-square test and multivariate logistic regression analysis. Analysis of SNPs and haplotypes in the IL10 and SNP in the ESR2 gene did not present any significant association with AgP or CP. The +2087G allele of the NOS2A gene tended to be significantly associated with periodontal disease. Patients carrying the genotype +2087GG in the NOS2A gene were genetically protected against the development of CP (p = 0.05; OR = 0.44; 95%CI = 0.20-0.95). This result showed greater significance when patients with AgP and CP were combined (total PD) (p = 0.03; OR = 0.46; 95%CI = 0.23-0.92). In conclusion, the studied Brazilian population had a significantly higher frequency of the GG genotype for the +2087 SNP in the NOS2A gene in individuals without periodontitis, although statistical significance was not maintained after multiple logistic regression.

Efeito antimicrobiano e modulador do óleo essencial extraído da casca de frutos da Hymenaea courbaril L / Antimicrobial effect and modulator of essential oil extracted from the fruits peel of Hymenaea courbaril L

O Jatobá (Hymenaea courbaril L.) possui um amplo histórico de utilização, seus frutos são compostos por óleos essenciais, taninos, substâncias amargas, matérias resinosas e pécticas, amido e açúcares, O objetivo deste estudo foi avaliar a atividade antimicrobiana e moduladora do óleo essencial extraído da casca de frutos da Hymenaea courbaril L, (OEHc) sobre cepas de S, aureus oxacilina sensíveis (OSSA) de referência, Para a avaliação da atividade antimicrobiana foram determinadas as Concentrações Inibitória (CIM) e Letal Mínimas (CLM) do OEHc e o efeito do tempo de exposição a concentrações do OEHc, o efeito modulador do OEHc sobre antibióticos de uso clínico foi avaliado pelo teste de difusão em ágar modificado, OEHc inibiu o crescimento das cepas de S, aureus ATCC 6538P (CIM = CLM = 0,28% v/v) e S, aureus ATCC 14458 (CIM = 0,28% v/v, CLM = 0,56% v/v), A CLM do OEHc foi capaz de inviabilizar as cepas OSSA testadas em 8 horas (S, aureus ATCC 6538P) e 24 horas (S, aureus ATCC 14458) e a CIM inibiu o crescimento de S, aureus ATCC 14458 até 48h de exposição, A ação moduladora do OEHc na atividade de antibióticos de uso clínico variou com o ATM e com a cepa testada, Os resultados encontrados mostram que o OEHc possui uma boa atividade antimicrobiana sobre a espécie Gram-positivo S, aureus, revelando seu efeito modulador sinérgico quando associado a antibióticos de uso clínico, demonstrando ser o OEHc um forte candidato para o desenvolvimento de fármacos com atividade antimicrobiana...

Jatobá (Hymenaea courbaril L.) has an extensive history of use, its fruits are composed of essential oils, tannins, bitter substances, resinous and pectic materials, starch and sugars. The objective of this study was to evaluate antimicrobial activity and effect of essential oil extracted from the rind of fruits of Hymenaea courbaril l. (OEHc) about reference strains of S. aureus oxacillin susceptible (OSSA). For the evaluation of antimicrobial activity were determined Inhibitory (MIC) and Lethal (CLM) Minimum Concentrations from OEHc and the effect of time of exposure to concentrations of OEHc, OEHc modulator effect on antibiotics of clinical use was assessed by modified agar diffusion test. OEHc inhibited the growth of S. aureus strains ATCC 6538P (CIM = CLM = 0,28% v/v) and S. aureus ATCC 14458 (CIM = 0,28% v/v; CLM = 0,56% v/v). The CLM from OEHc was able to derail the OSSA strains tested in 8 hours (S. aureus ATCC 6538P) and 24 hours (S. aureus ATCC 14458) and the CIM inhibited the growth of S. aureus ATCC 14458 until 48 hours of exposure. The modulatory action of the antibiotic activity of OEHc clinical use ranged with the ATM and with the strain tested. The results show that the OEHc has a good antimicrobial activity on the species Gram-positive S. aureus, revealing its synergistic modulator effect when associated with antibiotics of clinical use, demonstrating the OEHc a strong candidate for the development of drugs with antimicrobial activity...

Leukotoxicity of Aggregatibacter actinomycetemcomitans in generalized aggressive periodontitis in Brazilians and their family members

OBJECTIVE: The purpose of this study was to examine the leukotoxin promoter types of Aggregatibacter actinomycetemcomitans clones in subjects with generalized aggressive periodontitis (GAgP) and in their family members (FM). MATERIAL AND METHODS: Thirty-five patients with GAgP (33.9±7.1 years), 33 of their FM (22.8±11.4 years), and 41 patients with chronic periodontitis (CP) (44.1±9.4 years) were clinically analyzed using the plaque index, gingival index, probing depth (PD), and clinical attachment level (CAL). Subgingival biofilm samples were collected from four interproximal periodontal sites (>PD and >CAL) of each patient. The presence of A. actinomycetemcomitans and its leukotoxic clone was confirmed by polymerase chain reaction (PCR). RESULTS: A. actinomycetemcomitans was observed in 23 (51.1%) GAgP patients and 16 (30.1%) CP patients. Thirty-seven (94.8%) patients showed minimally leukotoxic strains and 2 (5.1%) showed highly leukotoxic strains. In the FM group, 10 (30.3%) had aggressive periodontitis (AgP), 12 (36.3%) had CP, 11 (33.3%) were periodontally healthy or had gingivitis, and 12.2% were A. actinomycetemcomitans positive. Greater full mouth PD and CAL were observed in GAgP patients positive for the bacteria than those negative for it (p<;0.05), and the presence of A. actinomycetemcomitans positively correlated with GAgP (Odds ratio, 3.1; confidence interval, 1.4-7.0; p=0.009). CONCLUSIONS: The presence of A. actinomycetemcomitans was associated with the clinical condition of GAgP, with most patients exhibiting a generalized form of the disease and minimally leukotoxic clones. Most of the relatives of GAgP patients presented either CP or AgP. .

Debridamento ultrassônico de boca toda no tratamento da periodontite crônica: uma revisão sistematizada / Full mouth ultrasonic debridement in the treatment of chronic periodontitis: a systematic review

Introdução: O debridamento ultrassônico de boca toda visa a uma instrumentação mais leve e conservadora da superfície radicular, evitando a recolonização da bolsa periodontal. Objetivo: Este trabalho verificou os efeitos do debridamento subgengival com o uso de ultrassom sobre os parâmetros clínicos periodontais no tratamento da periodontite crônica por meio de uma revisão na literatura. Material e Método: A revisão foi realizada nas bases de busca bibliográfica PubMed, Lilacs, Scielo, Medline e Centro de Registro de Ensaios Clínicos Controlados (Biblioteca Cochrane). Foram incluídas publicações na língua inglesa que abordaram estudos clínicos de intervenção em humanos, randomizados e controlados, e que utilizaram o debridamento ultrassônico para o tratamento não cirúrgico da periodontite crônica. Resultado: Inicialmente, foram encontradas 167 referências e, a partir da análise dos títulos e resumos, dez estudos perfizeram as características exigidas e foram considerados válidos para a realização desta revisão. Os estudos mostraram que a abordagem pelo debridamento ultrassônico pode ser terapia favorável ao tratamento da periodontite crônica. Conclusão: O tratamento da periodontite crônica por meio do debridamento ultrassônico periodontal de boca toda produz resultados clínicos semelhantes aos conseguidos na raspagem e no alisamento radicular convencionais.

Introduction: The full-mouth ultrasonic debridement seeks a more conservative instrumentation with light pressure of the root surface, preventing recolonization of periodontal pockets. Objective: This study examined the effects of subgingival debridement through the use of ultrasonic devices on clinical periodontal parameters in the treatment of chronic periodontitis by a review of the literature. Material and Method: Studies were selected on the bibliographic databases PubMed, Lilacs, Scielo, Medline and the Central Registry of Controlled Trials (The Cochrane Library). It was included English language publications with clinical studies of human intervention, randomized controlled trials that used ultrasonic debridement for the nonsurgical treatment of chronic periodontitis. Result: Initially, we found 167 references and analyzing the titles and abstracts totaled ten studies the characteristics required and were considered valid for the completion of this review. Studies have shown that the approach by ultrasonic debridement therapy may be favorable to the treatment of chronic periodontal disease. Conclusion: It can be concluded that the treatment of chronic periodontitis by full mouth periodontal ultrasonic debridement produces similar outcomes to those achieved in conventional scaling and root planing.

Latex constituents from Calotropis procera (R. Br.) display toxicity upon egg hatching and larvae of Aedes aegypti (Linn.)

Calotropis procera R. Br. (Asclepiadaceae) is a well-known medicinal plant with leaves, roots, and bark being exploited by popular medicine to fight many human and animal diseases. This work deals with the fractionation of the crude latex produced by the green parts of the plant and aims to evaluate its toxic effects upon egg hatching and larval development of Aedes aegypti. The whole latex was shown to cause 100 percent mortality of 3rd instars within 5 min. It was fractionated into water-soluble dialyzable (DF) and non-dialyzable (NDF) rubber-free materials. Both fractions were partially effective to prevent egg hatching and most of individuals growing under experimental conditions died before reaching 2nd instars or stayed in 1st instars. Besides, the fractions were very toxic to 3rd instars causing 100 percent mortality within 24 h. When both fractions were submitted to heat-treatment the toxic effects were diminished considerably suggesting low thermostability of the toxic compounds. Polyacrylamide gel electrophoresis of both fractions and their newly fractionated peaks obtained through ion exchange chromatography or desalting attested the presence of proteins in both materials. When submitted to protease digestion prior to larvicidal assays NDF lost most of its toxicity but DF was still strongly active. It may be possible that the highly toxic effects of the whole latex from C. procera upon egg hatching and larvae development should be at least in part due to its protein content found in NDF. However the toxicity seems also to involve non protein molecules present in DF.

Bactérias do gênero Salmonella em carcaças de frango comercializadas em Fortaleza, CE / Bacteria of the Salmonella commercialized carcasses of chicken in Fortaleza, CE

Com o objetivo de verificar a presença de bactérias do gênero Salmonella em amostras de carcaças de frango comercializadas na cidade de Fortaleza, e avaliar o potencial risco determinado pelo consumo desse produto, foram analisadas 66 carcaças de frango abtidas em estabelecimentos comerciais da cidade de Fortaleza-CE, sendo 46 (69,7 por cento) resfriadas e 20 (30,3 por cento) congeladas. As amostras foram transportadas sob refrigeração ao Setor de Microbiologia do Laboratório Central de Saúde Pública do Estado do Ceará (Lacen-CE) e submetidas à pesquisa de bactérias do gênero Salmonella, pelo método da lavagem superficial. Foi constatada a presença de bactérias do gênero Salmonella em 4,5 por cento das amostras analisadas, todas comercializadas sob refrigeração. Esses resultados confirmam estudos anteriores, que relatam a freqüente ocorrência de salmonelas em carcaças de frango, principalmente em carcaças resfriadas, e sugere a necesssidade de uma melhoria nas condições sanitárias das granjas e abatedouros locais.

Aspectos microbiológicos do queijo tipo "coalho" comercializado em Fortaleza - Ceará / Microbiological aspects of \"coalho\" type cheese commercialized in Fortaleza - Ceará

Avaliou-se a qualidade microbiológica de cinquenta e seis amostras de queijo tipo "coalho" provenientes de diferentes pontos comerciais de Fortaleza - Ceará. Foram quantificadas as bactérias mesófilas, Staphylococcus aureus, coliformes totais e fecais, bolores e leveduras. Verificou-se que em relaçäo às contagens de coliformes fecais e Staphylococcus aureus, 67,9 por cento e 62,5 por cento das amostras respectivamente, näo atenderam aos estabelecidos pela legislaçäo brasileira em vigor

Incidencia de tuberculose em pacientes aideticos / Incidence of tuberculosis in AIDS patients

Com a cosequente expansao da AIDS (Sindrome da Imunodeficiencia Adquirida), foram realizados estudos no sentido de investigar processos infecciosos em pacientes imunodeprimidos. A tuberculose, doença de grande prevalencia em nosso meio, tem despertado grande interesse. Inicialmente nao era considerada uma doença oportunista, no entanto vem se mostrando, assim como outras molestias, associada a muitos casos de AIDS. Este trabalho mostra a incidencia de tuberculose em pacientes aideticos, onde se investigou prontuario de 277 pacientes que tinham o diagnostico de AIDS, relacionado aos anos de 1988 a 2990. Destes 277 pacientes, 34(12,3%) foram diagnosticados com tuberculose, representando a terceira doença de maior incidencia associada a AIDS. A candidiase aparece em primeiro lugar com 275 caos, representando um percentual de 99,3% e a infecçao por criptococos em segundo lugar, com 230 (83%) dos casos. No ano de 1988 ocorreram 48 casos de AIDS, sendo 05 (10,4%) com tuberculose; no ano de 1989 surgiram 95 novos casos, sendo 11 (11,6%) com tuberculose, e no ano de 1990, 134 casos de AIDS, sendo 18 (13,4%) com tuberculose. A tuberculose pulmonar foi a forma clinica mais prevalente (seguida da glanglionar) com um percentual igual ou superior a 50%.

Estudo epidemiologico dos casos de Leishmaniose visceral (Calazar) registrados no Hospital Sao Jose, de 1980 a 1990 em Fortaleza-Ceara / Epidemiological study of Leishmania visceral cases occured on Sao Jose Hospital, Fortaleza, Ceara

Foi feito um levantamento dos prontuarios arquivados no Hospital Sao Jose (HSJ) no periodo de 1980 a 1990,dos casos de Leissssssshmaniose visceral (Calazar) no Ceara. Neste periodo foram registrados 170 casos de Calazar, sendo 163 (95,9%) receberam alta e 7 (4,1%) faleceram. Em 1980 e 1981 nao foi registrado nenhum caso de Calazar no HSJ. Os anos de 1985 e 1990 apresentaram o maior numero de casos, 30 (17,6%) e 39 (22,9%), respectivamente. Do total decasos notificados 137 (80,6%) procederam do interior e 33 (19,4%) da capital. Os meses de maior ocorrencia do Calazar foram em janeiro e setembro. A maior incidencia desta doença foi do sexo masculino com 129 (75,9%) casos e afaixa etaria mais atingida foi a de 16 a 30 anos com 71 (41,8%) casos. Todos os obitos registrados 7 (4,1%) foram de enfermos procedentes do interior.